EPIGENETICS

Epigenetics focuses on changes to DNA that don’t arise from sequence variants, but rather modifications to the physical and chemical nature of the sequence. Many modifications can affect whether a gene is activated or repressed. These include protein-DNA interactions, as well as nucleic acid modifications. To investigate these epigenetic markers, two common genomics approaches include chromatin immunoprecipitation (ChIP), and methylation studies, often referred to as bisulfite sequencing. Retrogen’s high-quality sequencing lab can help you with your epigenetic study.

To study protein-DNA interactions, one can cross link DNA to the proteins it interacts with, and then fragment and isolate these regions using an antibody specific to your protein of interest. Once isolated, the cross linking can be reversed, and the resulting fragments can be sequenced. Retrogen’s high-quality sequencing service can rapidly and effectively sequence your ChIP DNA helping you to progress your research. If interested in a large target area, then Retrogen’s ChIP-Seq service may be a better option for your project. Find more information on our PGM sequencing services here.

Unmethylated cytosine gets converted to uracil via bisulfite treatment, whereas methylated cytosine is resistant to this conversion under the same conditions. Using this simple principle along with standard sequencing allows interrogation of regions of interest and determination of the original methylation state in those regions.

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