GENOMIC SERVICES
Whole Plasmid Sequencing
Unlock the Full Potential of Plasmid Analysis
Whole plasmid sequencing has never been faster, more accessible, or more precise. Leveraging the power of Nanopore sequencing technologies, we provide a cutting-edge solution to fully characterize plasmids with unprecedented detail. Whether you’re in synthetic biology, gene therapy, or microbial research, our service delivers high-quality, end-to-end plasmid sequencing to accelerate your discoveries.
Why Choose Nanopore for Whole Plasmid Sequencing?
Nanopore sequencing revolutionizes plasmid analysis by offering unique advantages over traditional methods. With real-time data acquisition and long-read capabilities, this technology provides a comprehensive view of your plasmid’s structure and sequence—no assembly required.
Key Benefits:
- Complete Plasmid Coverage: Sequence entire plasmids in a single run, from small constructs to large, complex vectors.
- Long-Read Sequencing: Capture full-length reads up to tens of kilobases, eliminating gaps and ambiguities.
- Rapid Turnaround: Real-time sequencing reduces processing time, delivering results faster than ever.
- High Accuracy: Detect structural variants, insertions, deletions, and modifications with confidence.
- Cost-Effective: Streamlined workflows and scalable solutions tailored to your project size.
How It Works
Our whole plasmid sequencing service harnesses Nanopore’s innovative technology to provide a seamless experience from sample to insight.
- Sample Preparation: Submit your plasmid DNA, and we’ll handle the rest. Minimal input is required, with options for low- or high-throughput processing.
- Nanopore Sequencing: DNA passes through nanopores, generating electrical signals that are translated into sequence data in real time.
- Data Analysis: Receive fully assembled plasmid sequences, annotated features, and quality metrics through our intuitive platform.
- Custom Insights: Optional bioinformatics support to identify mutations, verify constructs, or compare against reference sequences.
Applications of Whole Plasmid Sequencing
Nanopore-based plasmid sequencing empowers a wide range of research and industrial applications:
- Synthetic Biology: Validate engineered plasmids for gene editing or protein expression.
- Gene Therapy: Ensure the integrity of therapeutic vectors, including AAV plasmids.
- Microbial Genomics: Study plasmid-mediated antibiotic resistance or horizontal gene transfer.
- Quality Control: Confirm plasmid identity and stability for biomanufacturing.
Why Partner with Us?
We combine state-of-the-art Nanopore sequencing with expert support to deliver reliable, actionable results. Our team is dedicated to advancing your research with:
- Industry-leading expertise in plasmid sequencing.
- Flexible solutions for academic, biotech, and pharmaceutical needs.
- Secure data handling and fast delivery of results.
Submission Requirements:
Our whole plasmid sequencing service requires you submit samples that meet quality and quantification requirements. The best results will come from whole, double stranded, clonal plasmids of high purity and an accurate concentration. Standard plasmid prep methods that have been performed correctly should be able to meet these requirements.
Purified plasmids should be in 10mM Tris of nuclease-free water.
A nanodrop reading with a 260/280 of <1.8 and a 260/230 of <2.0-2.2 indicates the sample is free from contaminants that will inhibit a successful sequencing run.
Visualizing the plasmid DNA on an agarose gel can confirm there is a single plasmid present at the expected size.
A fluorometric reader like the Qubit or equivalent give the most accurate reading of concentrations – a concentration based on other methods, such as spectrophotometers, may lead to a miss-estimation of DNA quantity.
Results Files:
a consensus .fasta file
a read length statistics and a pLannotate plot is presented to illustrate the polished plasmid consensus sequence.
a feature table containing annotations
Variant stats report – only if reference sequence is provided
Get Started Today
Ready to sequence your plasmids with unmatched precision? Contact us to discuss your project, request a quote, or learn more about how Nanopore technology can transform your workflow.
