GENOMIC SERVICES
Linear / Amplicon Sequencing
Long-Read Precision for Targeted Sequences
When you need full-length, phased, or modification-aware sequencing of specific regions, Nanopore linear and amplicon sequencing delivers unmatched clarity. Sequence 16S-ITS-23S full-length ribosomal operons, viral genomes, gene cassettes, HLA loci, or any custom amplicon up to 20 kb+ with zero gaps and native modification detection.
Why Choose Nanopore for Linear & Amplicon Sequencing?
Short-read methods fragment your target and lose phase and modification information. Nanopore reads your entire amplicon in one continuous read—preserving haplotypes, structural variants, and base modifications in real time.
Key Benefits:
- Full-Length Single Reads: Resolve complete targets (e.g., full-length 16S ~1.5 kb, 16S-ITS-23S ~5 kb, or larger constructs) in one read.
- Native Modification Detection: Detect 5mC, 6mA, and other epigenetic marks directly—no bisulfite needed.
- Multiplexing Power: Barcode hundreds of amplicons per run with native or PCR barcodes.
- Ultra-Fast Turnaround: From PCR product to final data in <24 hours with Rapid sequencing kits.
- Phasing & Variant Accuracy: Ideal for complex regions (repeats, GC-rich, phased SNPs/indels).
How It Works
- Amplicon Generation: Use your primers or let us design them.
- Library Prep: Ligation or Rapid PCR barcoding options available.
- Sequencing: Load and run on MinION or PromethION for depth tailored to your project.
- Analysis:Demultiplexing, consensus calling (medaka/racon), modification calling, and annotation.
Applications of Nanopore Linear / Amplicon Sequencing
- Full-Length 16S-ITS-23S Microbiome Profiling.
- Viral Genome Finishing & Quasispecies Analysis
- HLA Typing at Ultra-High Resolution.
- Phasing of Pathogenic Variants in Large Genes
- Synthetic Construct Verification (gBlock, Gene Fragments)
- CRISPR On/Off-Target Long-Read Validation
Submission Requirements:
Submit purified PCR products or cleaned amplicon pools. Minimum 50–100 ng total (depending on pool complexity). Products should be free of primers/dimers (recommended: bead cleanup or column purification). Provide primer sequences and expected sizes for optimal demultiplexing and analysis.
Results Files Delivered:
- Demultiplexed, high-accuracy consensus FASTA per barcode.
- Base modification (GFF/BED) reports.
- Read-length histogram and coverage plots.
- Consensus accuracy statistics (Q-score typically >Q30–Q50).
- Optional: Alignment to reference, variant calling, or phylogenetic placement
Get Started Today
Long-read amplicon sequencing has never been this accessible.
