RESOURCES
RETROGEN SEQUENCING TROUBLESHOOTING GUIDE AND SUGGESTIONS
How to order:
Prepare your samples using the submission template requirements at:
Universal primers, available at no additional charge, are listed here:
If you need custom primers synthesized, go to:
Log in to your dnaLIMS account at http://sequencing.retrogen.net and submit your order electronically.
- First-time Customers: Create a dnaLIMS account at: http://sequencing.retrogen.net/cgi-bin/dna/loginNn.cgi
- If you are within our pickup area, please schedule your pickup request through your dnaLIMS account (the pickup guidelines are below).
- Your results will be uploaded to the dnaLIMS system and you will receive a notification when they are available.
Packaging:
Single Tube Sequencing – Smaller orders should use 1.5mL Eppendorf (epi) tubes. Screw-cap tubes are more time-consuming, so pop-caps should be used instead. Each tube must be clearly labeled to match with the order forms. Tube strips, while convenient, are hard to label and keep track of, so please use 96-well plates for large orders (24+ samples).
96-Well Plate Sequencing – Please submit your DNA and primers at the concentration according to the tables on the “Sample Submission Guidelines” page. The concentration of DNA and primers should be normalized and arranged vertically (A1‐H1, A2‐H2, etc.) on a 96-well plate. The plate should be sealed using strip cap lids to prevent leakage and shipped overnight.
Shipping: Outside Southern California
Please ship your samples to 6645 Nancy Ridge Dr., San Diego, CA 92121. Samples may be shipped at ambient temperature. Dry ice is not necessary and the added weight increases shipping cost.
Free Sequencing Sample Pickup Service (Mon-Fri)
For customers in San Diego County, Orange County, and much of Los Angeles:
Please schedule your pickup request by telephone or electronically through your dnaLIMS account and leave your samples and order form at the pickup location (lab bench, front desk, drop box, etc.). Your results will be available online the following day by 10:00AM (12:00PM for Saturday results).
Please refer to the following guidelines for pickup times:
San Diego: La Jolla / Sorrento Valley
Please have your pickup scheduled and have your samples in the appropriate pickup location by 11:00AM for the first pickup. We will pick up your samples between 11:00AM and 1:00PM. For the second pickup, have your samples ready by 3:00PM and we will pick them up between 3:00PM and 5:00PM.
North San Diego County (North of Hwy 56)
Please have your pickup scheduled, electronically or by telephone, by 1:00PM and have your samples in the appropriate pickup location by 1:30PM. We will pick up your samples between 1:30 and 2:30PM and you will receive your results the following morning as usual.
San Diego (South of Hwy 52)
Please have your pickup scheduled, electronically or by telephone, and have your samples in the appropriate pickup location by 1:30PM. We will pick up your samples between 1:30 and 3:30PM and you will receive your results the following morning.
Orange County
Please have your pickup scheduled, electronically or by telephone, by 2:00PM and have your samples in the appropriate pickup location by 3:00PM. We will pick up your samples around 3:00PM and you will receive your results the following morning as usual.
Los Angeles County
Please have your pickup scheduled, electronically or by telephone, by 12:00PM and have your samples in the appropriate pickup location by 1:00PM. We will pick up your samples around 1:00PM and you will receive your results the following morning as usual. Please note: Los Angeles pickup area is limited to south of Burbank and west of Azusa. We currently do not offer pickup service in Diamond Bar, Pomona, or Claremont areas.
*For currently established pickup locations and drop boxes, please contact your sales representative.
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