RESOURCES
RETROGEN SEQUENCING TROUBLE SHOOTING GUIDE AND SUGGESTIONS
How To Order
Prepare your samples using the Submission Template Requirements at
Universal primers, available at no additional charge, are listed here:
If you need custom primers synthesized, go to:
Log in to your dnaLIMS Account at http://sequencing.retrogen.net and submit your order electronically.
- First-time Customers: Create a dnaLIMS account at http://sequencing.retrogen.net/cgi-bin/dna/loginNn.cgi
- If you are within our pickup area, please schedule your Pickup Request through your dnaLIMS account (the pickup guidelines are below).
- Your results will be uploaded to the dnaLIMS system and you will receive a notification when they are available.
Packaging:
Single Tube Sequencing – Smaller orders should use 1.5mL epi tubes. Screw cap tubes are more time consuming so pop caps should be used. Each tube must be clearly labeled to match with the order forms. Tube strips, while convenient, are difficult to label and to keep track of so for large orders (24+ samples) please use 96 well plates.
96 Well Plate sequencing – Please submit your DNA and primers at the concentration according to the table on the Submission Template Requirements page. The concentration of DNA and primers should be normalized and arrayed vertically from A1-H1, A2-H2…etc. on the 96-well plate. The plate should be sealed or capped using strip lids to prevent leakage and shipped overnight.
Shipping: Outside Southern California:
Please ship your samples to 6645 Nancy Ridge Dr., San Diego, CA 92121. Samples may be shipped at ambient temperature. Dry ice is not necessary and the added weight increases shipping cost.
Free Sequencing Sample Pickup Service (Mon-Fri):
San Diego County, Orange County, and much of Los Angeles
Please schedule your pickup request by telephone or electronically through your dnaLIMS account and leave your samples and order form in the pickup location (lab bench, front desk, drop box, etc.) Your results will be available online the following day by 10:00 am (12:00 pm for Saturday results). Please refer to the following guidelines for pickup times:
San Diego: La Jolla / Sorrento Valley
Please have your pickup scheduled and have your samples in the appropriate pickup location* by 11:00 am for the first pickup. We will pick up your samples between 11:00 am and 1:00 pm. For the second pickup have your samples ready by 3:00 pm and we will pick them up between 3:00 pm and 5:00 pm.
North San Diego County (North of Hwy 56)
Please have your pickup scheduled, electronically or by telephone, by 1:00 pm and have your samples in the appropriate pickup location* by 1:30 pm. We will pick up your samples between 1:30 and 2:30 and you will receive your results the following morning as usual.
San Diego (South of Hwy 52)
Please have your pickup scheduled, electronically or by telephone and have your samples in the appropriate pickup location* by 1:30 pm. We will pick up your samples between 1:30 – 3:30 and you will receive your results the following morning.
Orange County
Please have your pick up scheduled, electronically or by telephone by 2:00 pm, and have your samples in the appropriate pickup location* by 3:00 pm. We will pick up your samples around 3:00 pm and you will receive your results the following morning as usual.
Los Angeles County
Please have your pick up scheduled, electronically or by telephone by 12:00 pm, and have your samples in the appropriate pick up location* by 1:00 pm. We will pick up your samples around 1:00 pm and you will receive your results the following morning as usual. (Please note: Los Angeles pickup area is limited to south of Burbank and west of Azusa, we currently do not offer pick up service in Diamond Bar, Pomona, and Claremont areas)
*For currently established pickup locations and drop boxes, please contact your sales representative.