Industry-leading, gold-standard Sanger sequencing is performed on our ABI 3730xl platforms designed for high throughput without sacrificing quality. The high optical sensitivity and uniformity across a 96-capillary array, with the use of advanced polymers for separation, ensure that you receive the best quality sequencing results to support your applications.
We are equipped and experienced in the many complexities of Sanger sequencing, whether you need a single tube or a stack of plates to be sequenced. We are excited to expand your research under RUO or CLIA/CAP-regulated workflows.
Types of Service
Plasmids or PCR products
Provide your template and primer pre-mixed in a single well/tube according to our sample submission guidelines.
There are some templates where our proprietary sequencing reactions may be necessary to improve your sequencing results and avoid needing repeats. If your template is GC-rich, contains difficult repeats, hairpins and secondary structures or long homopolymers, then select our difficult template workflow (click here for more information).
Retrogen utilizes an enzymatic PCR cleanup method to remove unincorporated primers and dNTPs to ensure good-quality templates are available for your sequencing reactions.
CLIA/CAP-regulated workflows are available, allowing you to expand your clinical workflow and report Sanger sequencing results.
Clinical Sequencing Features & Benefits
Data routinely yielding long reads and quality above Q20.
Proprietary workflows enable sequencing the most difficult templates.
Local pickup available in select areas.
Quick Turnaround Time
Template Purification -> Sanger Sequencing Reaction -> Sequencing Cleanup -> Capillary Electrophoresis -> Data Analysis