Single-Reaction Sanger Sequencing

Sanger Sequencing for Single Targets

Labeled dideoxynucleotide termination of DNA strand growth, pioneered by Nobel laureate Frederick Sanger, has become the cornerstone of genomics. The ABI 3730 Genetic Analyzer utilizes lasers to illuminate the fluorescent dyes on the terminated strand. Single-reaction sequencing has expanded to massively parallel sequencing [a.k.a. next-generation sequencing (NGS)]. Illumina’s platforms use the same technology, but the fluorescence is read directly through a sensitive light meter. Thermofisher’s NGS technology now has a silicon chip with millions of pH meters built in, but doesn’t measure chain termination. Instead, it measures a H+ release from a growing strand, which is very different from Sanger sequencing. Although powerful, NGS covers full genomes or large DNA populations. Many researchers still need to focus on a small set of genes, thus Sanger sequencing on single targets is still a very viable technology in the lab, including clinical work.

Utilizing a single target and a single primer, fluorescent dideoxynucleotides terminate a growing strand of DNA in a thermal cycling reaction. After washing, the DNA population from the cycle sequencing reaction is separated by size through capillary electrophoresis, then the dideoxynucleotides are illuminated with a laser.

Special promotions!! **Promotions can be combined!!!**

    • Five free reactions! Write ‘New Customer, 5 Free’ in the comments section of your first order

    • 40% off for the first month!! Write “New Lab, 40% Off”

  • Competitive prices – call for a quote
  • Next-day turnaround
  • Monday-Friday results available by 10:00AM
  • Saturday results available by 12:00PM
  • Highly experienced genomics staff
  • Friendly technical support
  • Highest quality sequencing data
  • Up to 1100 base pairs per reaction
  • Consistent data from every run
  • Low template requirements
  • 17 universal primers available
  • Free repeats upon request
  • Go to http://sequencing.retrogen.net/
  • Select “Login” (top right)
  • Create a dnaLIMS account
  • Place an order
  • For a 5-reaction free trial, write “New Customer, 5 Free” or “New Lab, 40% off” in the comments section
  • Please include a printed copy of your order
  • For Southern California orders (south of Los Angeles), please contact Retrogen for pickup time and location

For more information, please call 1 (800) RETROGEN Sales - ext. 111 or 112